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KMID : 0369819960260010029
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Jorunal of Korean Pharmaceutical Sciences
1996 Volume.26 No. 1 p.29 ~ p.32
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Percutaneous Absorption of Recombinant h-EGF through Normal , Stripped and First-Degree Burn Skin
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조애리/Cho AR
이정욱/안병락/정주영/고여욱/심창구/Lee JU/Ahn BL/Chung JY/Koh YW/Shim CK
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Abstract
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In vivo and in vitro skin permeation of recombinant125 I-EGF through normal, stripped and the first degree burn skin were studied. The in vitro skin permeation rate through the first degree burn skin (296cpm/cm2/hr) and the stripped skin (1131cpm/cm2/hr) were 3.5 times and 13 times higher, respectively, as compared with the one through normal skin. In vivo absorption study with the first degree burn skin, the peak concentration of EGF in the skin was achieved at 1-3 hr and decreased afterward up to 8 hr with an elimination constant of 1.31×10?3g/ml/hr. To investigate the higher elimination rate of EGF in burn skin, binding and metabolism studies were conducted. No significant metabolism of EGF in burn skin (100?C,5?secondburning) was observed. With the presence or unlabelled-EGF 125I?EGF permeation through the burn skin showed higher permeation rate than the one without unlabelled-EGF. The result nay indicate that EGF-receptor binding play a role in determining the skin permeation rate.
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